没食子儿茶素没食子酸酯对硫酸镍诱导Beas-2B细胞氧化损伤的保护作用

Protective effect of EGCG on oxidative injury of Beas-2B cells induced bynickel sulfate

关键词: 表没食子儿茶素没食子酸酯（EGCG）  硫酸镍  Beas-2B细胞  氧化损伤

Keywords: fenvalerate  reproductive system  reproductive toxicity  pesticides

摘 要: 为了确定表没食子儿茶素没食子酸酯（EGCG）是否对人支气管上皮Beas-2B细胞具有抗氧化作用，本实验采用500 μmol/L硫酸镍诱导建立细胞氧化损伤模型，通过测定细胞存活率、ROS生成量、谷胱甘肽水平及丙二醛含量，探讨不同剂量（2.5、5、10 μmol/L）EGCG对硫酸镍诱导氧化损伤Beas-2B细胞的保护作用。结果表明，EGCG可以提高Beas-2B细胞的存活率，降低ROS生成量，提高GSH含量，降低MDA水平（P<0.05）。提示EGCG对Beas-2B细胞具有保护作用，其机制可能与减少细胞氧化损伤有关。

Abstract: Fenvalerate is a type Ⅱ pyrethroid pesticide with low toxicity and wide application range.Human and animal experiments have shown that it has some sperms damage effect and a certain [WTBZ]genetic[WTBZ] toxicity,besides,animal experiments also found it could induce interstitial cell damage,activity decrease of testicular markers enzyme,and feminization effect,etc.,the toxicity is related to exposure time and exposure dose.In female rats,fenvalerate can significantly inhibit the growth and development of preantral follicle and synthesis of follicular steroid hormone,interfer calcium homeostasis of ovarian cells,and suppress steroid synthesis of inferior ovarian granulosa cells by gonadotropin stimulation.This paper mainly reviewed the progress of researches on reproductive toxicity of fenvalerate,thereby provides some basis for future study.