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| 砷对不同组织来源细胞株毒性效应相关长链非编码RNA表达的影响 |
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杨净超,车旺军,陈斌,杨雪飞,段婷芝,杨欣达,何越峰
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1.昆明医科大学公共卫生学院,云南 昆明 650500; 2.昆明市疾病预防控制中心
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| 摘 要: 目的 探讨砷对乳腺、肝脏、肺细胞系长链非编码 RNA(LncRNA)GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON 表达的影响及其细胞特异性。方法 将处于对数生长期的人乳腺癌(MDA-MB-231)细胞系、人肝癌(HepG2)细胞系、人肺腺癌(A549)细胞系分别暴露于不同浓度的亚砷酸钠(NaAsO2)培养 72 h,检测 LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON 表达水平。结果 NaAsO2 能够促进 LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、DANCR、CCAT2、CCAT1、NEAT1、UCA1、FALEC 表达,抑制 LncRNA SPRY4-IT1 在 MDA-MB-231 细胞中的表达;促进 LncRNA GAS5、PCAT6、FENDRR、SPRY4-IT1、CCAT2、NEAT1、UCA1、FALEC、ABALON 表达,抑制 LncRNA HIF1A-AS1、DANCR、HEIH 在 HepG2 细胞中的表达;促进 LncRNA SPRY4-IT1、DANCR、NEAT1、FALEC、ABALON 表达,抑制 LncRNA GAS5、HIF1A-AS1、CCAT1、UCA1、HEIH 在 A549 细胞中的表达。结论 砷可影响 MDA-MB-231、HepG2、A549 细胞中 LncRNA GAS5、HIF1A-AS1、PCAT6、FENDRR、SPRY4-IT1、DANCR、CCAT2、CCAT1、NEAT1、UCA1、HEIH、FALEC、ABALON 的表达,且具有细胞特异性。 |
| 关键词: 砷 长链非编码 RNA(LncRNA) 特异性 |
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中图分类号: R994. 3
文献标识码: A
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| 基金项目: 云南省兴滇英才支持计划“医疗卫生人才”专项;昆明市春城计划高层次人才培养“春城名医”专项 |
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| Effect of arsenic on expression of long chain non-coding RNA associated to cell line toxic effects |
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YANG Jingchao, CHE Wangjun, CHEN Bin, YANG Xuefei, DUAN Tingzhi, YANG Xinda, HE Yuefeng
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School of Public Health, Kunming Medical University, Kunming, Yunnan 650500, China
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| Abstract: Objective To explore the effects of arsenic on the expression of LncRNA GAS5, HIF1A-AS1, PCAT6, FENDRR, SPRY4-IT1, DANCR, CCAT2, CCAT1, NEAT1, UCA1, HEIH, FALEC, ABALON, and its cell specificity. Methods Human breast cancer (MDA-MB-231) cell line, human liver cancer (HepG2) cell line, and human lung cancer (A549) cell line in logarithmic growth phase were cultured with different concentrations of sodium arsenite (NaAsO2) for 72 h. Then, detect the expression levels of LncRNA GAS5, HIF1A-AS1, PCAT6, FENDRR, SPRY4-IT1, DANCR, CCAT2, CCAT1, NEAT1, UCA1, HEIH, FALEC, and ABALON. Results The results showed that NaAsO2 could promote the expression of LncRNA GAS5, HIF1A-AS1, PCAT6, FENDRR, DANCR, CCAT2, CCAT1, NEAT1, UCA1, FALEC, inhibit the expression of LncRNA SPRY4-IT1 in MDA-MB-231 cells; promote the expression of LncRNA GAS5, PCAT6, FENDRR, SPRY4-IT1, CCAT2, NEAT1, UCA1, FALEC, ABALON, inhibit the expression of LncRNA HIF1A-AS1, DANCR, HEIH in HepG2 cells; promote the expression of LncRNA SPRY4-IT1, DANCR, NEAT1, FALEC, ABALON and inhibit the expression of LncRNA GAS5, HIF1A-AS1, CCAT1, UCA1, HEIH in A549 cells. Conclusion The results suggested that arsenic could affect the expression of LncRNA GAS5, HIF1A-AS1, PCAT6, FENDRR, SPRY4-IT1, DANCR, CCAT2, CCAT1, NEAT1, UCA1, HEIH, FALEC, ABALON in MDA-MB-231, HepG2, and A549 cells, and which was cell-specific. |
| Keywords: arsenic long chain non-coding RNA(LncRNA) specifity |
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