手传振动接触与血管活性因子表达水平的关联性分析

Correlation analysis between hand-transmitted vibration exposure and vasoactive factors expression level

关键词: 累计振动暴露水平(CVEL)  血管活性因子  内皮素(ET)  效应标志物  关联性

Keywords: cumulative vibration exposure level(CVEL)  vasoactive factor  endothelin(ET)  effect marker  correlation

摘 要: 目的 探讨手传振动接触与血清内皮素(ET)、一氧化氮(NO)、血栓素 A₂(TXA₂)、前列环素(PGI₂)表达水平的关联性。方法 采用典型抽样方法, 抽取广州市汽车制造业 190 名手传振动作业男工为振动组,210 名非手传振动作业男工为对照组。收集基本资料和代谢综合征(MetS) 相关指标, 检测 ET、 NO 和 TXA₂ 水解代谢产物血栓素 B₂(TXB₂)、PGI₂ 水解代谢产物 6-酮-前列腺素 F_1α(6-keto-PGF_1α)水平, 并将振动组男工按累计振动暴露水平(CVEL)分为低、中、高 3 个水平组。采用二元多因素 logistic 回归分析筛选血管活性因子的影响因素,采用 t 检验、F 检验和 LSD 法比较血管活性因子的表达水平。结果 调查对象的 ET、NO、TXB₂、6-keto-PGF_1α 检测结果分别为(119.50± 30.01)pg/ml、 (168.41±37.31)μmol/L、(92.00±23.15) pg/ml 和(754.75±203.34) pg/ml。 振动组的 ET 表达水平高于对照组(P<0.05),但振动组和对照组的 NO、TXB₂ 和 6-keto-PGF_1α 表达差异均无统计学意义(P>0.05)。CVEL 低、中、高水平组之间的 ET、NO、TXB₂ 和 6-keto-PGF_1α 表达差异均无统计学意义(P>0.05),但 CVEL 低水平组的 ET 表达水平高于中水平组(P<0.05)。 接触手传振动和罹患 MetS 均为 ET 表达水平的独立影响因素(OR_振动=1.54, OR_MetS=1.76, 均P<0.05)。无 MetS 的振动组 ET 表达水平高于对照组(P<0.05),低水平组 ET 表达水平高于中水平组(P<0.05)。 患 MetS 的振动组和对照组之间,CVEL 低、中、高水平组之间 ET 表达水平差异均无统计学意义(P>0.05)。结论 手传振动接触与血清 ET 表达水平有统计学关联。ET 可能为接触手传振动的效应标志物, 其发生机制尚需进一步探讨。

Abstract: Objective To explore the correlation between hand-transmitted vibration exposure and expression levels of endothelin (ET), nitric oxide (NO), thromboxane A2 (TXA₂) and prostacyclin (PGI₂) in serum. Methods Using typical sampling method, 190 male workers engaged in hand-transmitted vibration operation were selected as the vibration group, and 210 male workers engaged in non hand-transmitted vibration operation were selected as the control group in Guangzhou􀆳s automobile manufacturing industry. The study collected basic information and metabolic syndrome (MetS) related indicators, detected four indicators including ET, NO and hydrolysis metabolite thromboxane B₂ (TXB₂) of TXA₂ , hydrolysis metabolite 6-ketoprostaglandin F_1α(6-keto-PGF_1α) of PGI₂, and divided male workers from the vibration group into three levels: low, medium, and high, based on their cumulative vibration exposure level (CVEL). Binary multivariate logistic regression was used to screen the influence factors of vasoactive factors. Vasoactive factor expression levels were compared by t-test, F-test and LSD-method. Results The detection results of ET, NO, TXB₂ and 6-keto-PGF1α in the survey subjects were (119.50±30.01) pg/ml,(168.41±37.31) μmol/L,(92.00±23.15) pg/ml and (754.75±203.34) pg/ml, respectively. The ET expression level in vibration group was higher than that in control group (P<0.05), but there were no statistically significant difference in NO,TXB₂ and 6-keto-PGF_1α between vibration group and control group(P>0.05). There were no statistically significant difference in the expression of ET, NO, TXB₂ and 6-keto-PGF_1α among low-level group, medium-level group and high-level group (P>0.05), but the ET expression level in low-level group was higher than that in medium-level group (P<0.05). Exposure to handtransmitted vibration and suffering from MetS were both independent factors affecting ET expression level (OR_vibration=1.54,OR_MetS=1.76; P<0.05). For the survey subjects without MetS, the ET expression level in vibration group was higher than that in control group (P<0.05), and the ET expression level in low-level group was higher than that in medium-level group (P<0.05). However, for the survey subjects with MetS, there was no statistically significant difference in ET expression level between vibration group and control group, and there was no statistically significant difference among low-level group, medium-level group and high-level group (P>0.05). Conclusion The hand-transmitted vibration exposure was statistically associated with the ET expression level in serum. ET may be used as an effect biomarker for exposure to hand-transmitted vibration, but its underlying mechanism needs to be further explored levels of CYP1A1 protein in BMMNCs were significantly decreased (P<0.05). The above changes in BZ+Smoke group were more significant than those in BZ group (P<0.05). Conclusion The results suggested that benzene might induce hematotoxicity by activating the inflammatory signaling pathways of AhR and NLRP3, while the cigarette smoke might also increases the expression of AhR and NLRP3 proteins in liver tissues and bone marrow cells, thereby promoting the hematotoxicity of benzene.